Climate change is making it increasingly important to know how plants can survive and thrive in a changing environment. Conventional experiments in the lab have shown that plants accumulate pigments in response to environmental factors. To date, such measurements were made by taking samples, which required a part of the plant to be removed and thus damaged. “This labor-intensive method isn’t viable when thousands or millions of samples are needed. Moreover, taking repeated samples damages the plants, which in turn affects observations of how plants respond to environmental factors. There hasn’t been a suitable method for the long-term observation of individual plants within an ecosystem,” says Reiko Akiyama, first author of the study.
With the support of UZH’s University Research Priority Program (URPP) “Evolution in Action”, a team of researchers has now developed a method that enables scientists to observe plants in nature with great precision. PlantServation is a method that incorporates robust image-acquisition hardware and deep learning-based software to analyze field images, and it works in any kind of weather.
Using PlantServation, the researchers collected (top-view) images of Arabidopsis plants on the experimental plots of UZH’s Irchel Campus across three field seasons (lasting five months from fall to spring) and then analyzed the more than four million images using machine learning. The data recorded the species-specific accumulation of a plant pigment called “anthocyanin” as a response to seasonal and annual fluctuations in temperature, light intensity and precipitation.
PlantServation also enabled the scientists to experimentally replicate what happens after the natural speciation of a hybrid polyploid species. These species develop from a duplication of the entire genome of their ancestors, a common type of species diversification in plants. Many wild and cultivated plants such as wheat and coffee originated in this way.
In the current study, the anthocyanin content of the hybrid polyploid species A. kamchatica resembled that of its two ancestors: from fall to winter its anthocyanin content was similar to that of the ancestor species originating from a warm region, and from winter to spring it resembled the other species from a colder region. “The results of the study thus confirm that these hybrid polyploids combine the environmental responses of their progenitors, which supports a long-standing hypothesis about the evolution of polyploids,” says Rie Shimizu-Inatsugi, one of the study’s two corresponding authors.
PlantServation was developed in the experimental garden at UZH’s Irchel Campus. “It was crucial for us to be able to use the garden on Irchel Campus to develop PlantServation’s hardware and software, but its application goes even further: when combined with solar power, its hardware can be used even in remote sites. With its economical and robust hardware and open-source software, PlantServation paves the way for many more future biodiversity studies that use AI to investigate plants other than Arabidopsis – from crops such as wheat to wild plants that play a key role for the environment,” says Kentaro Shimizu, corresponding author and co-director of the URPP Evolution in Action.
The project is an interdisciplinary collaboration with LPIXEL, a company that specializes in AI image analysis, and Japanese research institutes at Kyoto University and the University of Tokyo, among others, under the Global Strategy and Partnerships Funding Scheme of UZH Global Affairs and the International Leading Research grant program of the Japan Society for the Promotion of Science (JSPS). The project also received funding from the Swiss National Science Foundation (SNSF).
Reiko Akiyama, Takao Goto, Toshiaki Tameshige, Jiro Sugisaka, Ken Kuroki, Jianqiang Sun, Junichi Akita, Masaomi Hatakeyama, Hiroshi Kudoh, Tanaka Kenta, Aya Tonouchi, Yuki Shimahara, Jun Sese, Natsumaro Kutsuna, Rie Shimizu-Inatsugi, Kentaro K. Shimizu: Seasonal pigment fluctuation in diploid and polyploid Arabidopsis revealed by machine learning-based phenotyping method PlantServation. Nature Communications. Doi: 10.1038/s41467-023-41260-3.